CHEMICALLY DEFINED MEDIUM FOR GROWTH OF STREPTOCOCCUS PYOGENES

Chemically Defined Medium for Growth Streptococcus Pyogenes.

Mickelson, M. N. (National Animal Disease Laboratory, Ames, Iowa). Chemically defined medium for growth of Streptococcus pyogenes. J. Bacteriol. 88:158-164. 1964.-Three strains of group A hemolytic streptococci representing three serological types were serially subcultured for an extended period (100 or more transfers) in a peptide-free and protein-free culture medium. An amino acid assay mediu...

Chemically defined medium for Legionella pneumophila growth.

A chemically defined medium containing 18 amino acids, inorganic salts, rhamnose, choline, and ferric pyrophosphate has been developed. The final concentrations of salts and amino acids were modeled after yeast extract. This medium supported the growth of four serogroups of Legionella pneumophila. Growth in shake cultures at 37 degrees C produced a lag time of approximately 5 h and a generation...

Requirements for growth of Streptococcus agalactiae in a chemically defined medium.

Chemically defined medium for growth and sporulation of Clostridium perfringens.

A chemically defined medium was developed that could support sporulation and growth of Clostridium perfringens strains ATCC 12916 and H9. This medium consisted of a modification of the basal medium of Boyd et al. plus 0.1% sodium thioglycolate and 0.5% monosodium glutamate. Five other strains grew, but did not sporulate, in this medium. With the addition of more vatamins into the medium, two mo...

Chemically defined medium for the growth of Pasteurella tularensis.

Chemically defined media for the growth of Pasteurella tularensis have been described by Tamura and Fleming (1949) and more recently by Giesken (1954), Mager et al. (1954), and Traub et al. (1955). Upon application of the latter medium (Traub et al.) for the growth of a virulent strain of P. tularensis, it was noted that the viable cell yields were markedly lower than yields previously obtained...